目录大肠杆菌英文的正确写法 大肠杆菌数英文 大肠杆菌英语音标 根治大肠杆菌最好的中药 大肠杆菌英语翻译
翻译
The Sanger double deoxidizing chain end, according to terminate method T7 Sequencing TM Kit instructions operation. [n a P]
DATP mark. 8% degeneration polyacrylamide gel, thickness of 0.4 mm, 55 cm long, electrophoresis glue in Pharmach LKB company Macrophor sequencing instrument, 1200 V ~ 1500 V, 2 ~ 5 hours. Sequencing product for x
The light of the negative light, in a critical 20 ℃ for the night, radiation from enhancement.
AT PJtts base solid cloning and expression will pPGAP-AT NcoI-SalI with double enzyme, insert pBV220 cut, get pBV220 a a1 AT a restructuring plasmid. Then one of the a1-AT (BamHl-Ava1) clips with the mutation PCR site-directed mutagenesis footage (BamHI-AvaI), that is, get base and replacement pBV220 purpose AT P [TTS expression plasmid. This
Plasmid into e. coli HB101, the medium in langmuir-blodgett 30 "C growth to mid logarithm. 42" C induction of three hours. Collect bacteria, ultrasonic broken respectively with 1 mol/after L, 2 mol/L urea washing, 8 mol/L urea dissolve
Inclusion body.
t2噬菌体的遗传物质是rna
t4噬菌体是dna。
噬颤亏菌体只是细菌病毒的统称，是以宿主种类划分的，起源不同，种类很多。
线状双链dna：大闭肌病毒、长尾病毒、短尾病毒、覆盖病毒等科。
环状双链dna：被脂病毒、原质病毒等科。
环状单链dna：微病毒、茄仿神丝状病毒等科。
线状双链rna：囊病毒科。
线状单链rna：光滑病毒科。
Using Sanger dideoxy chain termination method, according to the T7 Sequencing TM Kit usage instructions. [ n P]DATP markers. 8% denaturing polyacrylamide gel, gel thickness 0.4mm, long 55cm, electrophoresis in Pharmach LKB's Macrophor sequencing instrument, 1200V ~ 1500V, 2 ~ 5 hours. The sequencing of the X productLight negative exposure, in a 20 degrees overnight, autoradiography.AT PJtts based solid cloning and expression of pPGAP - AT NcoI - SalI double digestion, insert pBV220, pBV220 A1 AT recombinant plasmid. And then one of the a1-AT ( BamHl - Ava1 ) fragment with PCR site-directed mutagenesis of the mutant fragment ( BamHI - AvaI ) replacement, namely base base to pBV220 AT P [ TTS expression plasmid. ThePlasmid was transformed into E. coli HB101, in LB medium 30" C growth to the logarithmic term . 42" C induced by 3 hours. Lactobacillus collection, ultrasonic crushing respectively after using 1mol / L, 2mol / L urea washing, 8mol / L dissolving ureaInclusion body.
比较靠谱的翻译：
采用Sanger双脱氧链末端终止法，按T7 Sequencing TM Kit使用说明操作。[n一 P] dATP标记哗培搜。8% 变性聚丙烯酰胺凝胶，厚度0．4mm，胶长55cm，电泳在乱历Pharmach LKB公司的Macrophor测序仪上进行，1200V~1500V，2～5小时。测序产物置x光负片爆光，于一20℃过夜，放射自显影。
Sanger’s dideoxynucleotide chain terminating method was adopted and conducted according to the application instructions of T7 Sequencing TM Kit. Marked as [n一 P] dATP. The electrophoresis of 8%denaturing polyacrylamide gel with 0.4mm thickness and 55cm in length was conducted on Pharmach LKB Company’s Macrophor DNA sequencing machine at 1200V~1500V for 2～5 hours. The sequenced product was then subject to X-ray negative film explosion light and autoradiography for overnight at - 20°C.
AT PJtts基固克隆与表达: 将pPGAP —AT用NcoI—SalI双中首酶切，插入pBV220中，得到pBV220一a1一AT重组质粒。再将其中的a1-AT(BamHl—Ava1)片段用PCR定点诱变后的突变片段(BamHI—AvaI)置换，即得到垒基目的pBV220 AT P[tts表达质粒。
AT PJtts gene cloning and expression:NcoI—SalI double enzyme digestion was conducted on pPGAP —AT, and it was inserted into pBV220 vector to obtain pBV220一a1一AT recombinant plasmid. One of the a1-AT(BamHl—Ava1) gene fragments was displaced by (BamHI—Ava1) which was a mutated fragment from PCR site directed mutagenesis process, and thus barrier based purpose pBV220 AT P[tts expression plasmids were obtained.
该质粒转化大肠杆菌HB101，在LB培养基中30"C生长至对数中期．42"C诱导3小时。收集菌体，超声破碎后分别用1mol／L，2mol／L尿素洗涤，8mol/L尿素溶解
包涵体。
The plasmids were transformed into Escherichia coli HB101, they were cultured in LB medium at 30"C until mid-logarithmic growth and induced for 3 hours at 42"C. The thalli were collected and subject to ultrasonication; they were separately cleansed by using 1mol／L and 2mol／L urea, and then dissolved in 8 mol/L urea.
【英语牛人团】
yep，是全称“your educational plans”（个人教育计划）的缩略语。属于美语的俚语。与“yup”一样，属非正式场合及书面用语。表示肯定或赞成对方的口吻，也可以表示欣赏姿虚他人说法做法的口吻。
yup、yep、yes等词的联迹誉燃系与区别：
虽然yep、yup等词与yes的字面解释相同，但是使用yep、yup更加俏皮一些，适用于网络、通常会话中使用。而且多用于同辈人，朋友之间。请勿用于长辈，老师，因为yep、yup多少有点随意，不太尊虚巧重。
如果是在正式场合，请使用yes。yes属于正式场合及书面用语。
实际应用中，yep，yup跟yes在口语中有很大区别。 yep、yup均来源于印第安语与英语的演化。事实上，在美国中部，西部地区、加拿大、澳大利亚、新西兰口语中yep、yup常用于回答人家的感谢或表示肯定、赞成等；yep也是西方人回应Thank you的方式之一，而且流行程度远远超过You are welcome. 常见于餐厅，网络等非正式场合。正式场合中，yep一般不能替代You are welcome.[3].请不要过分依赖字典的字面意思，西方人不会用yep,yup代替yes。
